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How to Calibrate and use the pH Meter?

good morning guys so here in this video

I'm going to demonstrate to you how do

we do a calibration of power mantilla

Toledo pH meter so this is where the

instrumentation is and we need to have a

beaker this is the water and of course

do do calibration of pH meter in some pH

buffer right so before you do anything

make sure that your PPE is on make sure

you have your glass review your safety

goggles and of course lab coat webcoat

cover shoes right long pants the covers

and and socks that covers our ankles

alright so the buffer size you located

right in front of your birth over here

right so I've placed the buffer the

three of them makes examine them

carefully so we have the pH 4.01 and you

have the pH 9.2 1 and V 2 the buffer for

pH 7 alright and when is this solution

here this is actually your 3.0 molar KCl

which is used to store the finished

product take note that instead of pH

probe there must be a solution that you

have to submerge this even if not you

will deteriorate over time you notice

how this propria right you must always

put in to a free model KCl okay so

before we do any instrumentation

measurements for your sample we need to

do calibration right so press then turn

on you see it's already on now take out

this casing slowly just wrote it out be

rolling gentle this is quite fragile so

rotate out right rotate

alright then place on one side this part

here iph bro

we wash it first with DI water sprays on

their water at the prop here okay then

put the battery casing next we use a

piece of human wipe for in two halves

right

and then leave up the arm at this

position you put the arm and hover

around it just try it

bottom patches dab gently right right

and then next we press calibration so

this is Cal calibration press cow and

restart with pH 7 pH 7 right so that's

the buffer we will put the arm inside

there

now you notice that during this

calibration this prop here the sphere

mustn't have any bubbles around it so

you should be fully submerged into the

pH buffer and what do you do is you

watch the panel you'll see this right

here do you see this part here this in

here means it's still calibrating still

be waiting for result we see the circle

that means the instruments do doing its

job so just hold on if it's done you

will show another signal watch out for

this part now the best thing you do is

right actually do place the bar inside

here and I do not hold it because my

hands is warm so we know that pH is

affected by temperature change yeah you

see that this indication of temperature

so technologies temperature when you

measure your pH and also during

calibration all right so now you hear

the sound does this signal here the line

and stuff flashing and blinking that

means it's done so use pH so now I did

that okay of course get back your

solution put it back inside the stand

and of course same thing again you

repeat the washing because there's some

stains of the Prius buffer of the pH so

just spray around okay and next I'll use

a piece of keen white go into - yeah and

just wipe around your eye capsule that

on the probe okay do not use too much

force you know it's too much force but

if you very gentle please next you go

for pH for

same thing put the arm down okay if you

see this bubble try this surrounding the

probe you have to shake this vial to

allow the bubbles will come up so I do

not want to give some heat to the

solution I will just leave it stand

right you find it is not stable you can

actually use another beaker a small

beaker to hold it in place

so so now you press Cal calibration tool

against detecting now meanwhile let me

just show you these two vowels right the

big one of course this pH probe can see

in science I've shown you but what about

the small one the small is able to just

nice make sure that a pH probe enters it

of course if you have some bubbles here

that means you cannot shake it there's

no space for them to just get off the

bubble so we advise you to just stick to

the larger bow this kind right so again

let's wait for it

before this pH to be calibrated

okay now you hear that sound right good

so pH 4.0 and s exactly the same pH as

what it's supposed to be good so again

do the same thing twelve the arm gently

and then tap your buffer solution put it

back to the stand and the repetition of

the step see again so rinse it with the

deionized

why we're up you Fenian space okay this

pH probe is flexible the arm is so be

very careful okay we can tell good so

next we'll test the last pH calibration

pH nine point two one open the cap push

down the arm okay

and look out for bubbles it shouldn't be

any bubbles here all the way down move

your hands away so there's no heat yeah

and after that press calibration again

okay good nine point two one this time

is very fast it is all done so good now

remove the probe from the pH solution

okay put it back to the stand and before

you measure the pitch of the sample

again don't forget to rinse it with

distilled water deionized water

all right so why that's okay

hope you're a customer this is red

etiquette okay good so now let's add a

sample of solution

I want to measure so this is all done

right we click exit okay good

again not a temperature change just now

what's twenty four point nine hours 24

pee now this is my unknown solution I'm

going to place it right my ph probe

inside there okay

make sure the liquid doesn't spill over

it doesn't flow over

okay and then once i'm ready i will

click read this part the neon green Rick

so sleep acting again get back for this

signal to confirm the exact th very

accurate make sure that this is not

flashing before you can take down the

reading there should be a sound but if

it likes too noisy just watch out for

this flashing a you see now happens the

temperature increased so Oh a signal of

the temperature that you record your pH

reading note the data so in time this pH

meter try to use it for homogeneous

solution if you have some solid is

recommended not to use it because you

have some solid that stains on the

exterior surface of the pH probe that

can affect the reading but let's say

unfortunately you have inductive there's

some solid honest on the surface of this

pH probe please inform the engineer cut

down in a log book so that we know what

to follow up for okay and after

performing any usage of this instrument

you can always have to record on it's

not here just place right above these

are range logbook pH meter right open it

read instructions activity column the in

so calibration usage maintenance so in

this case I get a calibration of course

demonstration so today state is the 9th

of September 2016 time is time is 8:48

a.m. this is calibration calibration

plus demonstration

I put video okay but it's not just now

so that means now the reading is

recorded this is not flashy anymore

finish of my unknown solution in 6.01

okay good so in this case you see that

now this is icon here the since thought

they beans right you taste your

measuring menu pH you can copy down

because your hands lost weight you can

start it first so this reading will be

safe and later you can even export this

data by using a thumb drive at the back

here there's a USB port in the back of

this pH meter

okay so my username and this is okay

alright okay

very good and see the data you can

actually export to USB stick at the back

there that's it just now so after you're

done please put this book back over here

then if you're still not sure there are

instructional manuals over here these

three different languages no excuse we

can read them and most importantly after

you have mastered it you need to do some

housekeeping right rinse the pH probe

with bi water once more so that the next

user we have it clean right we do not

want to see our pH probe and pH meter to

be dirty when we use it is it right so

again you gentle I wipe it and what's

next you have to put it back to the

three molar KCl to preserve it okay so

here just

rigor in make sure there's no bubbles is

Lisa much so if you need to top up the

solution in the future please you know

what you get it from here

alright this then we put back D buffer

in this location if we are done for the

day you can turn off the pH meter but

let's say you're at the midday noon time

you do not have to turn it off because

it takes about half an hour to restart

the machine again so the next user can

just do humidity stay safe and enjoy